Pubblicazioni di GIORGIA GIOACCHINI

56 pubblicazioni disponibili classificate nel seguente modo:

Nr. doc. Classificazioni
55 1 Contributo su Rivista
1 8 Tesi di dottorato

Anno Risorsa
2017 Effects of BPA on female reproductive function: The involvement of epigenetic mechanism
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Autore/i: Santangeli, Stefania; Maradonna, Francesca; Olivotto, Ike; Piccinetti, Chiara Carla; Gioacchini, Giorgia; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246443 Collegamento a IRIS

2017 Integrated control of fish metabolism, wellbeing and reproduction: The role of probiotic
AQUACULTURE
Autore/i: Carnevali, Oliana; Maradonna, Francesca; Gioacchini, Giorgia
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246444 Collegamento a IRIS

2017 Host-probiotic interaction: new insight into the role of the endocannabinoid system by in vivo and ex vivo approaches
SCIENTIFIC REPORTS
Autore/i: Gioacchini, Giorgia; Rossi, Giacomo; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: The endocannabinoid system plays an important role in regulating inflammation in several chronic or anomalous gut inflammatory diseases. In vivo and ex vivo studies showed that 30 days treatment with a probiotic mix activated the endocannabinoid system in zebrafish. These results highlight the potential of this probiotic mixture to regulate immune cell function, by inducing gene expression of toll-like receptors and other immune related molecules. Furthermore, TUNEL assay showed a decrease in the number of apoptotic cells, and this finding was supported by a reduction in pro-apoptotic factors and an increase in anti-apoptotic molecules. The results presented here strengthen the molecular mechanisms activated by probiotic mix controlling immune response and inflammation.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246826 Collegamento a IRIS

2017 Effects of diisononyl phthalate on Danio rerio reproduction
ENVIRONMENTAL POLLUTION
Autore/i: Santangeli, Stefania; Maradonna, Francesca; Zanardini, Maya; Notarstefano, Valentina; Gioacchini, Giorgia; Forner-Piquer, Isabel; Habibi, Hamid; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: Di-isononyl phthalate (DiNP) is a high molecular weight phthalate commonly used as a plasticizer. It was introduced as a replacement for bis (2-ethylhexyl) phthalate (DEHP) which is used in the production of plasticized polyvinyl chloride (PVC). The purpose of this study was to investigate for the first time the effect of DiNP on female reproductive physiology in Danio rerio. Fish were exposed to five different doses of DiNP plus control (0 μg/L; 0.42 μg/L; 4.2 μg/L; 42 μg/L; 420 μg/L; 4200 μg/L) for a period of 21 days. We evaluated fish fecundity, oocyte growth, autophagic and apoptotic processes, as well as changes in morphological and biochemical composition of oocytes, using, qPCR analysis, histology and Fourier transform infrared imaging. The results demonstrate a non-monotonic dose response to DiNP. Greater differences were observed at the lowest (0.42 μg/L) and higher concentrations (420 μg/L; 4200 μg/L) of DiNP. The findings provide evidence that exposure to DiNP adversely affect oocytes growth and maturation, leading to abnormal gonadal development and reproduction in zebrafish.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251109 Collegamento a IRIS

2016 Probiotic treatment reduces appetite and glucose level in the zebrafish model
SCIENTIFIC REPORTS
Autore/i: Falcinelli, Silvia; Rodiles, Ana; Unniappan, Suraj; Picchietti, Simona; Gioacchini, Giorgia; Merrifield, Daniel Lee; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: The gut microbiota regulates metabolic pathways that modulate the physiological state of hunger or satiety. Nutrients in the gut stimulate the release of several appetite modulators acting at central and peripheral levels to mediate appetite and glucose metabolism. After an eight-day exposure of zebrafish larvae to probiotic Lactobacillus rhamnosus, high-throughput sequence analysis evidenced the ability of the probiotic to modulate the microbial composition of the gastrointestinal tract. These changes were associated with a down-regulation and up-regulation of larval orexigenic and anorexigenic genes, respectively, an up-regulation of genes related to glucose level reduction and concomitantly reduced appetite and body glucose level. BODIPY-FL-pentanoic-acid staining revealed higher short chain fatty acids levels in the intestine of treated larvae. These results underline the capability of the probiotic to modulate the gut microbiota community and provides insight into how the probiotic interacts to regulate a novel gene network involved in glucose metabolism and appetite control, suggesting a possible role for L. rhamnosus in the treatment of impaired glucose tolerance and food intake disorders by gut microbiota manipulation.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/229807 Collegamento a IRIS

2016 A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla
JOURNAL OF FISH BIOLOGY
Autore/i: Mazzeo, I; Giorgini, E; Gioacchini, G; Maradonna, F; Vílchez, M C; Baloche, S; Dufour, S; Pérez, L; Carnevali, O; Asturiano, J F
Classificazione: 1 Contributo su Rivista
Abstract: A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic process, such as ovary histology, fat content analysis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and vitellogenin enzyme linked immunosorbent assay (ELISA), a new technique, Fourier-transform infrared (FT-IR) microspectroscopy, was used to analyse A. anguilla ovaries. The results from the different techniques provided different ways of approaching the same process. Although it is considered a time consuming approach, of all the employed techniques, histology provided the most direct evidences about vitellogenesis. SDS-PAGE and ELISA were also useful for studying vitellogenesis, whereas fat analysis cannot be used for this purpose. The FT-IR analysis provided a representative IR spectrum for each ovarian stage (previtellogenic stage, early vitellogenic stage, mid-vitellogenic stage and late vitellogenic stage), demonstrating that it is a valid method able to illustrate the distribution of the oocytes within the ovary slices. The chemical maps obtained confirmed changes in lipid concentrations and revealed their distribution within the oocytes at different maturational stages. When the results and the accuracy of the FT-IR analysis were compared with those of the traditional techniques commonly used to establish the vitellogenic stage, it became evident that FT-IR is a useful and reliable tool, with many advantages, including the fact that it requires little biological material, the costs involved are low, analysis times are short and last but not least, the fact that it offers the possibility of simultaneously analysing various biocomponents of the same oocyte.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/236683 Collegamento a IRIS

2016 Characterization of Ambra1 in asexual cycle of a non-vertebrate chordate, the colonial tunicate Botryllus schlosseri, and phylogenetic analysis of the protein group in Bilateria
MOLECULAR PHYLOGENETICS AND EVOLUTION
Autore/i: Gasparini, Fabio; Skobo, Tatjana; Benato, Francesca; Gioacchini, Giorgia; Voskoboynik, Ayelet; Carnevali, Oliana; Manni, Lucia; Valle, Luisa Dalla
Classificazione: 1 Contributo su Rivista
Abstract: Ambra1 is a positive regulator of autophagy, a lysosome-mediated degradative process involved both in physiological and pathological conditions. Nowadays, Ambra1 has been characterized only in mammals and zebrafish. Through bioinformatics searches and targeted cloning, we report the identification of the complete Ambra1 transcript in a non-vertebrate chordate, the tunicate Botryllus schlosseri. Tunicata is the sister group of Vertebrata and the only chordate group possessing species that reproduce also by blastogenesis (asexual reproduction). B. schlosseri Ambra1 deduced amino acid sequence is shorter than vertebrate homologues but still contains the typical WD40 domain. qPCR analyses revealed that the level of B. schlosseri Ambra1 transcription is temporally regulated along the colonial blastogenetic cycle. By means of similarity searches we identified Wdr5 and Katnb1 as proteins evolutionarily associated to Ambra1. Phylogenetic analyses on Bilateria indicate that: Wdr5 is the most related to Ambra1, so that they may derive from an ancestral gene, ii) Ambra1 forms a group of ancient genes evolved before the radiation of the taxon, iii) these orthologous Ambra1 share the two conserved WD40/YVTN repeat-like-containing domains, and iv) they are characterized by ancient duplications of WD40 repeats within the N-terminal domain.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228483 Collegamento a IRIS

2016 BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction
SCIENTIFIC REPORTS
Autore/i: Santangeli, Stefania; Maradonna, Francesca; Gioacchini, Giorgia; Cobellis, Gilda; Piccinetti, Chiara Carla; Dalla Valle, Luisa; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression levels of genes related to reproduction are caused by histone modifications, BPA concentrations consistent with environmental exposure were administered to zebrafish for three weeks. Effects on oocyte growth and maturation, autophagy and apoptosis processes, histone modifications, and DNA methylation were assessed by Real-Time PCR (qPCR), histology, and chromatin immunoprecipitation combined with qPCR analysis (ChIP-qPCR). The results showed that 5 μg/L BPA down-regulated oocyte maturation-promoting signals, likely through changes in the chromatin structure mediated by histone modifications, and promoted apoptosis in mature follicles. These data indicate that the negative effects of BPA on the female reproductive system may be due to its upstream ability to deregulate epigenetic mechanism.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/233338 Collegamento a IRIS

2016 Bisphenol A Induces Fatty Liver by an Endocannabinoid-Mediated Positive Feedback Loop
ENDOCRINOLOGY
Autore/i: Martella, Andrea; Silvestri, Cristoforo; Maradonna, Francesca; Gioacchini, Giorgia; Allarà, Marco; Radaelli, Giuseppe; Overby, Darryl R; Di Marzo, Vincenzo; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: The xenoestrogen bisphenol A (BPA) is a widespread plasticizer detectable within several ecosystems. BPA is considered a metabolic disruptor, affecting different organs; however, little is known about its mechanism of action in the liver, in which it triggers triglyceride accumulation. Adult zebrafish (Danio rerio) exposed to BPA developed hepatosteatosis, which was associated with an increase in the liver levels of the obesogenic endocannabinoids 2-arachidonoylglycerol and anandamide and a concomitant decrease in palmitoylethanolamide. These changes were associated with variations in the expression of key endocannabinoid catabolic and metabolic enzymes and an increase in the expression of the endocannabinoid receptor cnr1. Acute and chronic in vitro treatments with nano- and micromolar BPA doses showed increased anandamide levels in line with decreased activity of fatty acid amide hydrolase, the main anandamide hydrolytic enzyme, and induced triglyceride accumulation in HHL-5 cells in a CB1-dependent manner. We conclude that BPA is able to produce hepatosteatosis in zebrafish and human hepatocytes by up-regulating the endocannabinoid system.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/235628 Collegamento a IRIS

2015 Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis
THERIOGENOLOGY
Autore/i: Vílchez, M Carmen; Santangeli, Stefania; Maradonna, Francesca; Gioacchini, Giorgia; Verdenelli, Cristina; Gallego, Victor; Peñaranda, David S; Tveiten, Helge; Pérez, Luz; Carnevali, Oliana; Asturiano, Juan F
Classificazione: 1 Contributo su Rivista
Abstract: Positive effects of probiotics on fish reproduction have been reported in several species. In the present study, 40 male European eels were weekly treated with recombinant hCG for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) CFU/mL) of probiotic Lactobacillus rhamnosus IMC 501 (Sinbyotec, Italy). The probiotics were daily added to the water from the sixth week of the hCG treatment. Males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of probiotic treatment (seventh-ninth weeks of hCG treatment); at this point, sperm and testis samples were also collected. Sperm volume was estimated, and motility was analyzed by computer-assisted sperm analysis software. Alternations in transcription of specific genes involved in reproductive process such as activin, androgen receptors α and β (arα and arβ), progesterone receptor 1 (pr1), bone morphogenetic protein 15 (bmp15), and FSH receptor (fshr) were analyzed in the testis. After 2 weeks of probiotic treatment, sperm production and sperm motility parameters (percentage of motile cells and percentage of straight-swimming spermatozoa) were increased in the European eel treated with 10(5) CFU/mL compared to controls or to the other probiotic doses. These changes were associated with increases in messenger RNA expression of activin, arα, arβ, pr1, and fshr. Conversely, after 3 weeks, activin and pr1 expression decreased. No significant changes were observed on bmp15 expression throughout the duration of the treatment with 10(5) CFU/mL dose. The lowest and highest probiotic dose (10(3) and 10(6) CFU/mL, respectively) inhibited the transcription of all genes along all the experiment, except for arα and arβ after 1 week of probiotic treatment when compared to controls. The changes observed by transcriptomic analysis and the sperm parameters suggest that a treatment with L rhamnosus at 10(5) CFU/mL for 2 weeks could improve spermatogenesis process in Anguilla anguilla.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/227825 Collegamento a IRIS

2015 Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes.
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Autore/i: Miccoli, A; Gioacchini, G; Maradonna, F; Benato, F; Skobo, T; Carnevali, O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/224974 Collegamento a IRIS

2015 Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism
SCIENTIFIC REPORTS
Autore/i: Falcinelli, Silvia; Picchietti, Simona; Rodiles, Ana; Cossignani, Lina; Merrifield, Daniel L; Taddei, Anna Rita; Maradonna, Francesca; Olivotto, Ike; Gioacchini, Giorgia; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (fit2, agpat4, dgat2, mgll, hnf4α, scap, and cck) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism, and reveals a new potential role for L. rhamnosus in the treatment of lipid disorders.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/226112 Collegamento a IRIS

2014 VIBRATIONAL CHARACTERIZATION OF FEMALE GAMETES: A COMPARATIVE STUDY
ANALYST
Autore/i: E. GIORGINI; G. GIOACCHINI; S. SABBATINI; C. CONTI; L. VACCARI; A BORINI; O. CARNEVALI; G. TOSI
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/184105 Collegamento a IRIS

2014 A new approach to evaluate aging effects on human oocytes: Fourier transform infrared (FTIR) imaging spectroscopy study.
FERTILITY AND STERILITY
Autore/i: Gioacchini G; Giorgini E; Vaccari L; Ferraris P; Sabbatini S; Bianchi V; Borini A; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/128301 Collegamento a IRIS

2014 A developmental hepatotoxicity study of dietary bisphenol A in Sparus aurata juveniles.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C. TOXICOLOGY & PHARMACOLOGY
Autore/i: Maradonna F; Nozzi V; Dalla Valle L; Traversi I; Gioacchini G; Benato F; Colletti E; Gallo P; Di Marco Pisciottano I; Mita DG; Hardiman G; Mandich A; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/183902 Collegamento a IRIS

2014 Alkylphenolic contaminants in the diet: Sparus aurata juveniles hepatic response
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Autore/i: Traversi, I.; Gioacchini, G.; Scorolli, A.; Mita, D.G.; Carnevali, O.; Mandich, A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/229910 Collegamento a IRIS

2014 The influence of probiotics on zebrafish danio rerio innate immunity and hepatic stress.
ZEBRAFISH
Autore/i: Gioacchini G; Giorgini E; Olivotto I; Maradonna F; Merrifield DL; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/159108 Collegamento a IRIS

2014 Melatonin-mediated effects on killifish reproductive axis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR & INTEGRATIVE PHYSIOLOGY
Autore/i: Lombardo F; Gioacchini G; Fabbrocini A; Candelma M; D'Adamo R; Giorgini E; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/159109 Collegamento a IRIS

2014 Dietary modulation of immune response and related gene expression profiles in mirror carp (Cyprinus carpio) using selected exotic feed ingredients
AQUACULTURE
Autore/i: M.D. Rawlinga; ; D.L.Merrifield; H.Kühlwein; D. Snellgrove; G.Gioacchini; O. Carnevali ; S.J.Davies a
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/143078 Collegamento a IRIS

2013 Assay of vtg, ERs and PPARs as endpoint for the rapid in vitro screening of the harmful effect of Di-(2-ethylhexyl)-phthalate (DEHP) and phthalic acid (PA) in zebrafish primary hepatocyte cultures
TOXICOLOGY IN VITRO
Autore/i: Francesca Maradonna; Matteo Evangelisti ; Giorgia Gioacchini; Beatrice Migliarini ; Ike Olivotto; Oliana Carnevali
Classificazione: 1 Contributo su Rivista
Abstract: In the last years the concern about the negative effects of phthalates on reproduction significantly increased. Considering that, at date data available dealing with the adverse outcome of Di-(2-ethylhexyl)- phthalate (DEHP) on the reproduction of several species are still contrasting, in this study, the effects induced by DEHP (0.05, 0.1, 1, 10 and 100 nM) and its active metabolite, phthalic acid (PA) (0.01, 0.1, 1 and 10 lM), were analyzed in zebrafish, Danio rerio, primary hepatocyte cultures, using target molecules involved in fish reproduction (vitellogenin – vtg and estrogen receptors – ERa, b1 and b2) and metabolism (peroxisome proliferators activated receptors – PPAR a, b, c). The use of in vitro culture, in fact, has the potential to significantly reduce the number of animals sacrificed for research allowing a precise control of the physical and chemical parameters that is often not possible in vivo. Moreover, since many toxicological studies revealed a sex specific response to toxicants, male and female primary hepatocyte cultures were set up to elucidate the possible gender specific effects of two common environmental phthalates. The increase of vtg levels observed in the culture media of male or female hepatocytes strongly evidenced the phthalates E2-like action. Moreover, the data obtained suggested that the observed different ERs isoforms modulation is otherwise associated with the vtg increase, depending on fish gender. Regarding PPARs, a similar trend of expression was found in both males and females. In conclusion, this study enforces the role of vtg as biomarker for evaluate the presence of environmental doses of DEHP and PA. Considering the similar gender modulation observed for vtg and PPARs, these molecules could be used for the rapid screening of the presence of DEHP and PA. Noteworthy the gender specific modulation observed for ERs opens a debate on the estrogenic mechanism of action of DEHP and PA and their role on vtg induction.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82838 Collegamento a IRIS

2013 Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity.
FISH & SHELLFISH IMMUNOLOGY
Autore/i: Abid A; Davies SJ; Waines P; Emery M; Castex M; Gioacchini G; Carnevali O; Bickerdike R; Romero J; Merrifield DL.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/128482 Collegamento a IRIS

2013 Expression of 8-OHdG in Zosterisessor ophiocephalus from the Venetian lagoon, Italy
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Autore/i: Negrato E, Vascellari M; Capolongo F ] ; Binato G ; Da Dalt L; Papo MB ; Gioacchini G; Carnevali O] ; Bertotto D ; Radaell G.; Pascoli, Pascoli F
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/100065 Collegamento a IRIS

2013 Probiotic Pediococcus acidilactici modulates both localised intestinal- and peripheral-immunity in tilapia (Oreochromis niloticus).
FISH AND SHELLFISH IMMUNOLOGY
Autore/i: Standen BT; Rawling MD; Davies SJ; Castex M; Foey A; Gioacchini G; Carnevali O; Merrifield DL
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/128489 Collegamento a IRIS

2013 Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic.
REPRODUCTION FERTILITY AND DEVELOPMENT
Autore/i: Gioacchini G; Valle LD; Benato F; Fimia GM; Nardacci R; Ciccosanti F; Piacentini M; Borini A; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87414 Collegamento a IRIS

2013 Probiotic supplementation promotes calcification in Danio rerio larvae: a molecular study
PLOS ONE
Autore/i: Francesca Maradonna; Giorgia Gioacchini; Silvia Falcinelli; Daniela Bertotto; Giuseppe Radaelli; Ike Olivotto; Oliana Carnevali
Classificazione: 1 Contributo su Rivista
Abstract: A growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. We previously demonstrated that dietary supplementation with Lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in Danio rerio (zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of key genes involved in ossification, e.g. runt-related transcription factor 2 (runx2), Sp7 transcription factor (sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate (gla) protein (bglap) as well as through inhibition of sclerostin (sost), a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3-(Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation, documented an increase in Mapk1 16 days post fertilization (dpf) and of Mapk3 23 dpf in individuals receiving L. rhamnosus supplementation. Interestingly, a reduction of sost detected in the same individuals suggests that the probiotic may help treat bone disorders.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/143077 Collegamento a IRIS

2013 Ambra1 knockdown in zebrafish leads to incomplete development due to severe defects in organogenesis.
AUTOPHAGY
Autore/i: Benato F; Skobo T; Gioacchini G; Moro I; Ciccosanti F; Piacentini M; Fimia GM; Carnevali O; Dalla Valle LD.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87413 Collegamento a IRIS

2013 Effects of probiotic administration on zebrafish development and reproduction
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Autore/i: Carnevali O; Avella MA; Gioacchini G
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/100066 Collegamento a IRIS

2012 The role of melatonin on Zebrafish follicle development: an FT-IR imaging approach
VIBRATIONAL SPECTROSCOPY
Autore/i: E. Giorgini; G. Gioacchini; C. Conti; P. Ferraris; S. Sabbatini; G. Tosi; C. C. Piccinetti; L. Vaccari; O. Carnevali
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82205 Collegamento a IRIS

2012 Are Acartia tonsa cold stored eggs a suitable food source for the marine ornamental species Amphiprion polymnus? A feeding study.
AQUACULTURE NUTRITION
Autore/i: Ike Olivotto ; Gioia Gaiot 1; Linda Holste ; Francesca Tulli ; Gloriana Cardinaletti; Chiara Carla Piccinetti ; Giorgia Gioacchini; Oliana Carnevali.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/76951 Collegamento a IRIS

2012 Probiotics can induce follicle maturational competence: the Danio rerio case
BIOLOGY OF REPRODUCTION
Autore/i: GIOACCHINI G; GIORGINI E; MERRIFIELD D L; HARDIMAN G; BORINI A; VACCARI L; CARNEVALI O
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/65072 Collegamento a IRIS

2012 Melatonin effects on Fundulus heteroclitus reproduction.
REPRODUCTION FERTILITY AND DEVELOPMENT
Autore/i: Lombardo F; Giorgini E; Gioacchini G; Maradonna F; Ferraris P; Carnevali O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/77346 Collegamento a IRIS

2011 Effects of Probiotic on Zebrafish Reproduction.
JOURNAL OF AQUACULTURE RESEARCH & DEVELOPMENT
Autore/i: GIOACCHINI G; LOMBARDO F; MERRIFIELD D L; SILVI S; CRESCI A; AVELLA M A; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/65076 Collegamento a IRIS

2011 Perspectives on endocrine disruptor effects on metabolic sensors
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Autore/i: MIGLIARINI B. ; PICCINETTI C.C. ; MARTELLA A.; MARADONNA F. ; GIOACCHINI G.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54458 Collegamento a IRIS

2011 FOCAL PLANE ARRAY (FPA) FOURIER TRANSFORM INFRARED (FT-IR) IMAGING SPECTROSCOPY AS A NEW TECHNIQUE TO EVALUATE OF HUMAN OOCYTE QUALITY.
PLACENTA
Autore/i: Gioacchini G; Carnevali O; Giorgini E; Vaccari L; Bianchi V; Borini A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83167 Collegamento a IRIS

2011 Melatonin Induces Follicle Maturation in Danio rerio.
PLOS ONE
Autore/i: Carnevali, O; Gioacchini, G; Maradonna, F; Olivotto, I; Migliarini, B
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/56781 Collegamento a IRIS

2011 Evaluation Of Human Oocytes Ageing By Focal Plane Array (FPA) Fourier Transform Infrared (FT-IR) Imaging Spectroscopy.
FERTILITY AND STERILITY
Autore/i: GIOACCHINI G; CARNEVALI O; GIORGINI E; VACCARI L; BIANCHI V; BORINI A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83498 Collegamento a IRIS

2011 Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection.
FISH AND SHELLFISH IMMUNOLOGY
Autore/i: Pérez-Sánchez T; Balcázar JL; Merrifield DL; Carnevali O; Gioacchini G; de Blas I; Ruiz-Zarzuela I.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/56784 Collegamento a IRIS

2011 Effects of probiotic on zebrafish (Danio Rerio) reproduction
Editore: Università Politecnica delle Marche
Classificazione: 8 Tesi di dottorato
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/241887 Collegamento a IRIS

2010 Welfare improvement using alginic acid in rainbow trout (Oncorhynchus mykiss) juveniles
CHEMISTRY IN ECOLOGY
Autore/i: GIOACCHINI G.; LOMBARDO F.; AVELLA M.A.; OLIVOTTO I.; CARNEVALI O.
Editore: Editore Gordon and Breach Science Publishers Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/39838 Collegamento a IRIS

2010 Expression of VTG and CYP1A biomarkers in Zosterisessor ophiocephalus sampled in different areas of the Venice Lagoon
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR & INTEGRATIVE PHYSIOLOGY
Autore/i: NEGRATO E. ; PASCOLI F. ; BERTOTTO D. ; RADAELLI G.; MARADONNA F.; GIOACCHINI G.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54463 Collegamento a IRIS

2010 Oocytes maturation induction by Lactobacillus rhamnosus in Danio rerio: in vivo and in vitro studies
HUMAN REPRODUCTION
Autore/i: GIOACCHINI G.; BIZZARO D.; GIORGINI E.; FERRARIS P.; SABBATINI S.; CARNEVALI O
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54494 Collegamento a IRIS

2010 Melatonin control of oogenesis and metabolic resources in Zebrafish
JOURNAL OF APPLIED ICHTHYOLOGY
Autore/i: CARNEVALI O.; GIOACCHINI G.; PICCINETTI C.C.; MARADONNA F.; LOMBARDO F.; GIORGINI E.; TOSI G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54460 Collegamento a IRIS

2010 Application of multi-species of Bacillus in sea bream larviculture
AQUACULTURE
Autore/i: AVELLA M.A.; GIOACCHINI G.; DECAMP G.; MAKRIDIS P.; CARNEVALI O.
Editore: Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/29247 Collegamento a IRIS

2010 Could probiotics improve fecundity? Danio rerio as case of study.
JOURNAL OF BIOTECHNOLOGY
Autore/i: GIOACCHINI G.; GIORGINI E.; FERRARIS P.; TOSI G.; BIZZARO D.; SILVI S.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54461 Collegamento a IRIS

2010 Effects of Lactobacillus rhamnosus on zebrafish oocyte maturation: an FTIR imaging and biochemical analysis
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Autore/i: GIORGINI E.; CONTI C.; FERRARIS P.; SABBATINI S.; TOSI G.; RUBINI C.; VACCARI L.; GIOACCHINI G.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Abstract: The aim of this study was to verify the effects of probiotic Lactobacillus rhamnosus on zebrafish oocyte maturation using FPA (focal plane array) FTIR imaging together with specific biochemical assays (SDS–PAGE, real-time PCR and enzymatic assay). Oocyte growth is prevalently due to a vitellogenic process which consists of the hepatic synthesis of vitellogenin and its selective uptake during maturation. The administration of L. rhamnosus IMC 501 for 10 days induced chemical changes to oocyte composition, promoting the maturation process. Some interesting biochemical features, linked to protein secondary structure (amide I band) and to phospholipidic and glucidic patterns, were detailed by vibrational analysis. The spectroscopic results were supported by the early increase of the lysosomal enzyme involved in the final oocyte maturation, the cathepsin L. This enzyme increases during follicle maturation, with the highest levels in class IV oocytes. In treated females, class III oocytes showed higher cathepsin L gene expression and enzymatic activity, with levels comparable to class IV oocytes isolated from controls; this can be related to the proteolytic cleavage of the higher molecular mass yolk protein components, as evidenced by SDS–PAGE.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54459 Collegamento a IRIS

2010 Increase of fecundity by probiotic administration in zebrafish (Danio rerio). Reproduction. 2010 Sep 10. [Epub ahead of print] PubMed PMID: 20833753.
REPRODUCTION
Autore/i: GIOACCHINI G; MARADONNA F; LOMBARDO F; D. BIZZARO; OLIVOTTO I; CARNEVALI O
Editore: Portland Press Limited:Commerce Way, Colchester CO2 8HP Essex United Kingdom:011 44 1206 796351, EMAIL: sales@portland-services.com, INTERNET: http://www.portland-services.com, Fax: 011 44 1206 799331
Classificazione: 1 Contributo su Rivista
Abstract: 2010 Sep 10. [Epub ahead of print] PubMed PMID: 20833753
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/34302 Collegamento a IRIS

2010 Increase of fecundity by Probiotic administration in zebrafish (Danio rerio)
REPRODUCTION
Autore/i: GIOACCHINI G. ; MARADONNA F.; LOMBARDO F.; BIZZARO D.; OLIVOTTO I.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54475 Collegamento a IRIS

2009 FT-IR Microspectroscopy on molecular building of Zebrafish oocytes
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: CARNEVALI O; CONTI C; FERRARIS P; GARAVAGLIA MG; GIOACCHINI G; GIORGINI E; RUBINI C; SABBATINI S; TOSI G
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51257 Collegamento a IRIS

2008 Effects of Ergosan on early immune response of rainbow trout (Oncorhynchus mykiss) juveniles to enteric red mouth (ERM) vaccine
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Autore/i: GIOACCHINI G.; SMITH P.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54534 Collegamento a IRIS

2008 Changes in cathepsin gene expression and relative enzymatic activity during gilthead sea bream oogenesis
MOLECULAR REPRODUCTION AND DEVELOPMENT
Autore/i: CARNEVALI O.; CIONNA C.; TOSTI L.; CERDA' J.; GIOACCHINI G.
Editore: John Wiley & Sons Incorporated:Customer Service, 111 River Street:Hoboken, NJ 07030:(800)225-5945, (201)748-6000, EMAIL: societyinfo@wiley.com, INTERNET: http://www.wiley.com, Fax: (212)748-6551
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/31048 Collegamento a IRIS

2008 The use of harpacticoid copepods as live prey for Amphiprion clarkii larviculture: Effects on larval survival and growth
AQUACULTURE
Autore/i: OLIVOTTO I.; CAPRIOTTI F.; BUTTINO I.; AVELLA M.; GIOACCHINI G.; MARADONNA F.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50710 Collegamento a IRIS

2007 The role of fatty acids enrichments in the larviculture of false percula clownfish Amphiprion ocellaris
AQUACULTURE
Autore/i: AVELLA M.A.; OLIVOTTO I.; GIOACCHINI G.; MARADONNA F.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52301 Collegamento a IRIS

2006 Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression
AQUACULTURE
Autore/i: CARNEVALI O.; DE VIVO L.; SULPIZIO R.; GIOACCHINI G.; OLIVOTTO I.; SILVI S.; CRESCI A.
Classificazione: 1 Contributo su Rivista
Abstract: In the present study a LacticAcidBacteria (LAB) strainLactobacillus delbrueckii delbrueckii, acting as probiotic, was administered toDicentrarchus labrax(European sea bass) juveniles for a short (25 days) and a long (59 days) time and the effects of the bacteria on gut colonization in sea bass juveniles cortisol level and growth were evaluated with respect to fish fed on live preys solely (control group). Measures of body weight and standard length (S.L.) were performed to assess juveniles growth, while cortisol, a hormone directly involved in stress responses, was chosen as a stress marker. In addition, the expression of two antagonistic genes involved in muscular growth (IGF-I andmyostatin (MSTN) was analyzed through real-time PCR. Fish fed on live preys addedwithL. delbrueckii delbrueckii showed a high gut colonization by this Lactic Acid Bacteria strain. In the same fish a significantly (pb0.05) lower cortisol level was observed with respect to those fed on live preys solely (control 5.1±0.47 ng/g; group A: 3.6±0.36 ng/g; group B: 4.5±0.48 ng/g). In addition, an increase of IGF-I transcription was observed in fish treated with LAB, being IGF-I mRNA levels six times higher in both treated groups with respect to the control (control: 1±0.01 a.u.; group A: 6.63±0.18 a.u.; group B: 6.16±0.15 a.u.). On the contrary, MSTNmRNA transcriptionwas significantly inhibited in treated groups (control 1±0.02 a.u.; groupA: 0.71±0.01 a.u.; groupB: 0.26± 0.003 a.u.). These results are in agreement with the increase of bodyweight recorded in this study. Fish fed on LAB showed 81%higher body weight in long treated group and 28% in short treated one with respect to control.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/31332 Collegamento a IRIS

2005 Hormonal control of the IGFs system in the sea bream ovary
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Autore/i: GIOACCHINI G.; CARDINALI M.; MARADONNA F.; FUNKENSTEIN B.; MOSCONI G.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54489 Collegamento a IRIS

2004 Hormonal regulation of vasa-like mRNA expression in the ovary of the marine teleost Sparus aurata
BIOLOGY OF REPRODUCTION
Autore/i: CARDINALI M.; GIOACCHINI G.; CANDIANI S.; PESTARINO M.; CARNEVALI O.
Editore: Society for the Study of Reproduction:1619 Monroe Street:Madison, WI 53711:(608)256-2777, EMAIL: ssr@ssr.org, INTERNET: http://www.ssr.org, Fax: (608)256-4610
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/31038 Collegamento a IRIS

2004 Cloning and expression of high choriolytic enzyme, a component of the hatching enzyme system, during the embryonic development of the marine ornamental fish Chrysiptera parasema
MARINE BIOLOGY
Autore/i: OLIVOTTO I.; YASUMASU S.; GIOACCHINI G.; MARADONNA F.; CARNEVALI O.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52500 Collegamento a IRIS





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